In vitro anticancer, antioxidant and DNA-binding study of the bioactive ingredient of clove and its isolation

Cancer cannot be ignored since it is the most dangerous disease because a major cause of death globally with 15% mortality. Researchers have been attracted to plant-based solution this havoc. Among all plants, Syzygium aromaticum has shown tremendous results in many aspects such as anticancer, antioxidant, and others. All studies that took place, were done on plant extract only. No one goes further than this. Hence, an advanced computational chemistry-based method for characterization identification bioactive ingredients isolated from cloves was developed first time. First, different extracts buds obtained using solvents (Water, methanol, chloroform, ethyl acetate, 50% ethanol, hexane), then each evaluated its anticancer activity against A549 H1299 lung cancer cell lines. The fractionation active flash chromatography. After that, evaluation every fraction again. One fractions showed highest activity. For experimental IR NMR data taken compared 19 compounds found cloves. Furthermore, DNA binding affinity antioxidant showing also studied. presented isolation ingredient will helpful scientists working field separation science phytomedicine.